DISTRIBUTION OF PROTEINASE IN CULTURES OF A SPECIES OF MICROCOCCUS IN SYNTHETIC MEDIUM,
Abstract
Cultures of Micrococcus sp. (ATCC No. 407) in synthetic medium contained proteinase giving optimum casein hydrolysis at pH 5.7-6.3. The proteinase (named pH 5.7 proteinase) was found in the supernatant fluid of centrifuged cultures; the amount in the medium increased during the log phase of growth and reached a maximum just before cultures entered the maximum stationary phase. The occurrence of pH 5.7 proteinase in the external medium did not seem to be associated with cell lysis. Cells harvested at intervals during growth of cultures contained a small, nearly constant amount of pH 5.7 proteinase suggesting the enzyme is liberated by cells at about the same rate as it is formed. No evidence was obtained to suggest that cells contained inactive proenzyme or precursor. Cells contained peptidase activity and pH 7.0 proteinase (optimum casein hydrolysis at pH 7.0). These enzymes were liberated when cells were ruptured and apparently are produced by cells independently of pH 5.7 proteinase. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 27, 1965
- Accession Number
- AD0621618
Entities
People
- Ian J. McDonald
Organizations
- National Research Council Canada