NATURE OF CYTOTOXIC REACTIONS MEDIATED BY ANTIBODY AND COMPLEMENT, AND RELATED PHENOMENA.
Abstract
It has been possible to distinguish and physically separate two stages in the lysis of sensitized erythrocytes by complement, i.e., (1) a step involving interaction of complement with the sensitized cell, and (2) a subsequent step which appears to be an intrinsic cellular reaction. While the complement reaction (step 1) can be blocked by chelating agents such as citrate of ethylene diamine tetraacetate, as well as by competing complement-fixing antigen-antibody systems, the intrinsic reaction (step 2) is not arrested by these agents, nor by washing of sensitized cells following treatment with complement, nor by chilling to 0C. The intrinsic process has a low temperature coefficient (Q sub 10 about 1.5) and it proceeds at an appreciable rate even at 0C. Measurements conducted at 37C indicate that the intrinsic cellular reaction requires about 2-3 minutes per cell. This time interval, however, does not account adequately for the lag period, and it appears likely that the complement reaction proper (step 1) involves a series of 2 or more reaction steps. Evidence obtained in studies on the role of Ca(++) and Mg(++) in immune hemolysis supports this conclusion. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 15, 1953
- Accession Number
- AD0621645
Entities
People
- Manfred M. Mayer
Organizations
- Johns Hopkins University