LABELLING OF HUMAN FIBRINOGEN WITH IODINE131 BY ELECTROLYTIC IODINATION,
Abstract
An original method for labelling proteins with radioactive I is proposed, and its application to the 131I iodination of human fibrinogen is illustrated. The method is based on the electrolysis of a solution of K131I in presence of the protein to be labelled. The labelling procedure and the electrolytic cell are described in detail. The advantages in comparison with the traditional techniques are 3-fold: (a) there is no need of oxidizing agents; (b) the possibility exists of controlling the rate of liberation of I; and (c) there is the possibility of using extremely dilute solutions of KI, thus reducing the amount of I introduced into the molecule for a given specific activity. The product thus obtained does not show modifications of its original characteristics, as judged from electrophoresis in agar and from the values of the 'turnover-time' which are obtained in a group of normal human subjects. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 26, 1963
- Accession Number
- AD0621787
Entities
People
- F. Pennisi
- G. A. Scassellati
- N. Riccioni
- P. Giagnoni
- U. Rosa