THIN-LAYER CHROMATOGRAPHY OF SOME 131-I AND 125-I LABELLED IODOPYRIMIDINES AND RELATED NUCLEOSIDES,

Abstract

The separation of some radioiodinated pyrimidines and nucleosides of biological interest was studied by thin-layer chromatography. Rf values of iodouracil, iodocytosine, iodouridine, iododeoxyuridine and iodocytidine on layers of silica gel G and cellulose G were measured for a number of solvents: n-butanol saturated with water, water, n-butanol saturated with 0.1 N H3BO3, n-butanol saturated with i N H3BO3, a saturated solution of H3BO3 in water, 0.1 N HCOOH, n-butanol saturated with 0.1 N HCOOH and n-butanol saturated with o.i N NH4OH were used. With a proper choice of elution system and support, a very good resolution of the single spots can be attained and the relative percentages of the labelled components in a sample can be evaluated with a negligible error by radioactive scanning of the chromatographic plate. (Author)

Document Details

Document Type
Technical Report
Publication Date
Jun 22, 1964
Accession Number
AD0621814

Entities

People

  • A. Massaglia
  • S. Sosi
  • U. Rosa

Tags

DTIC Thesaurus Topics

  • Cellulose
  • Chemical Compounds
  • Chromatography
  • Gels
  • Nucleosides
  • Pyrimidines
  • Scanning
  • Silica Gels
  • Silicon Dioxide
  • Thin Layer Chromatography

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Electrochemical Engineering/ Fuel Cell Technologies
  • Molecular and Cellular Biochemistry