A FLUOROMETRIC PROCEDURE FOR MEASURING THE ACTIVITY OF DEHYDROGENASES.
Abstract
A simple, direct, fluorometric method is described for measuring the activity of dehydrogenases. The method is based upon the conversion of the nonfluorescent material resazurin to the highly fluorescent compound resorufin, in conjunction with the nicotinamide adenine dinucleotide-reduced nicotinamide adenine dinucleotide (NAD(+)-NADH) system. By the procedures described, approximately 0.00010 to 0.1000 unit/ml of lactic acid dehydrogenase, alcohol dehydrogenase, malic acid dehydrogenase, glutamic acid dehydrogenase, glucose6-phosphate dehydrogenase, L-alphs-glycero phosphate dehydrogenase (GPDH), and glycerol dehydrogenase (GDH) may be determined with standard deviations of approximately =1.0%. Similarly, diaphorase, 0.000400 to 0.0800 unit/ml, resazurin, 0.00000001 to 0.00001 M, and NADH, 0.0000002 to 0.00002 M, may be determined with standard deviations of =0.5%, =0.8%, and =0.5%, respectively. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1965
- Accession Number
- AD0622341
Entities
People
- David N. Kramer
- George G. Guilbault
Organizations
- Edgewood Chemical Biological Center