EFFECT OF EXCHANGE TRANSFUSION ON INCORPORATION OF THYMIDINE-H3 INTO DEOXYRIBONUCLEIC ACID AND ON MITOSES IN HEPATOCYTES OF NORMAL AND REGENERATING RAT LIVER,
Abstract
Exchange transfusion of blood (75 to 80 percent replacement of blood of recipient animals) was used to study the effect of blood-borne influences on labeling of DNA and occurrence of mitoses in hepatocytes of rat liver. Intact or sham-hepatectomized rats and partially hepatectomized rats were (1) not transfused, (2) transfused with blood from rats with intact livers, or (3) transfused with blood from rats with partly excised livers. Partially hepatectomized or sham-hepatectomized or sham-hepatectomized rats were transfused once or twice (consecutively) at intervals after operation. Non-hepatectomized rats were transfused at equivalent times before they were killed. Incorporation of thymidine-H3 was detected autoradiographically and mitoses were counted in histological sections. The following effects on labeling of DNA and occurrence of mitoses in hepatocytes were found: (1) no change in rats with intact livers transfused with blood from donor rats with either intact or partly excised livers, (2) depression or delay in partially hepatectomized rats transfused with blood from donor rats with intact livers (delay prolonged by two consecutive transfusions), and (3) a variable depression or delay in partially hepatectomized rats transfused with blood from partially hepatectomized donor rats (the extent of depression of the peak rate of DNA labeling normally occurring at 24 hours after hepatectomy was directly related to the mass of residual liver in donor rats). (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 23, 1965
- Accession Number
- AD0622805
Entities
People
- G. F. Leong
- J. D. Emerson
- J. W. Grisham
- M. L. Albright
Organizations
- Naval Radiological Defense Laboratory