ELECTROCHEMISTRY OF ENZYMES. DETERMINATION OF PEROXIDASE AND CATALASE.
Abstract
Two representative enzyme systems, peroxide-catalase and peroxide-peroxidase, were used in the development of an electrochemical method for their determination. An electrochemically active substrate was oxidized to two electroinactive products by proper control of the current and experimental conditions. A proton donor with nonelectroactive oxidation and reduction forms was used with peroxidase to evolve its enzymatic function. The donor was not electroactive at the potentials used. With this procedure, 0.0100 to 1.00 unit of peroxidase or catalase was determined with a relative standard deviation of 1.7%. The potentials used in the described method were studied by anodic polarographic techniques to determine their significance. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1965
- Accession Number
- AD0623004
Entities
People
- George C. Guilbault
Organizations
- Edgewood Chemical Biological Center