THE JUVENILE HORMONE-I. METHODS OF PURIFICATION AND ISOLATION,
Abstract
Methods for the purification and isolation of junvenile hormone from Hyalophora cecropia are described. Ether extraction, low temperature precipitation, thin layer chromatography, and gas liquid chromatography were utilized. JH activity was followed by means of the Tenebrio bioassay. An apparent gain in activity of approximately five-fold was obtained during the course of purification. This was interpreted to be largely the result of a loss of inhibitors during the low temperature precipitation procedure. The final active material, indicated to be a single compound by preliminary gas liquid chromatographic analysis, represented 0.00450.0051 per cent of the crude oil used as starting material. The juvenile hormone activity was increased from 25 Tenebrio Units per microliter for the crude oil to approx. or = 2.6 x 10 to the 6th power Tenebrio Units per microliter for the final active substance. This represents an approximate 1.05 x 10 to the 5th power-fold purification. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 23, 1965
- Accession Number
- AD0623178
Entities
People
- H. Roller
- J. S. Bjerke
- W. H. Mcshan
Organizations
- University of Wisconsin–Madison