HOMOGRAFT RESPONSE AND HEMAGGLUTININ PRODUCTION BY SENSITIZED THYMECTOMIZED IRRADIATED ADULT MICE,
Abstract
Thymectomized and nonoperated male B6D2F1 mice were sensitized by the following means: (1) an allogeneic (A/HeJ) skin graft; (2) three sc injections of A/HeJ spleen cells in Freund's complete adjuvant; and (3) three ip injections of A/HeJ spleen cells. One week after the last injection, thymectomized and nonoperated mice from each group received 870 rad whole-body X radiation. The mice were grafted with A/HeJ and rat skin 102, 143, 184, 205 and 225 days after irradiation. Sera were obtained frequently for anti-A/HeJ and anti-rat hemagglutinins. The results indicated that although both hemagglutinin production and the homograft response were initially greatly impaired by thymectomy and lethal irradiation, repeated antigenic challenge resulted in return of the specific homograft response to near normal reactivity while antibody production became progressively more impaired. The results suggested that (1) antibody production and homograft sensitivity are manifestations of immunologic responses by associated by distinct cell populations, (2) antibody production is more thymus-dependent than is the homograft response and (3) the method of sensitization employed determines both the relative and absolute number of 'sensitized cells' produced within each cell population. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 13, 1965
- Accession Number
- AD0624485
Entities
People
- Leonard J. Cole
- Marvin L. Tyan
Organizations
- Naval Radiological Defense Laboratory