ACTIVE IMMUNIZATION VS. COCCIDIOIDES IMMITIS

Abstract

Cellular, sonicated and autoclaved-sonicated antigens derived from arthrospores and from spherules were tested by the complement fixation method against cooled sera obtained from rabbits infected with C. immitis. These tests were performed to detect antigenic differences between arthrospores and spherules. An attempt also was made to correlate these results with previous data obtained by testing similar antigens with immune rabbit sera. An angar-gel double diffusion assay was performed to demonstrate the number of precipitin bands present in soluble extracts derived from arthrospores and from spherules, and the relationship of these precipitin bands among the extracts. The indirect immuno-fluorescent assay was utilized to confirm the antigenicity of the cell wall of arthrospores and of spherules, and of their articulate fractions.

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Document Details

Document Type
Technical Report
Publication Date
Jul 31, 1965
Accession Number
AD0624511

Entities

People

  • Norman F. Conant

Organizations

  • Duke University

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Animals
  • Antibodies
  • Cells
  • Cellular Structures
  • Diffusion
  • Dilution
  • Fluorescence
  • Immune Serums
  • Lagomorphs
  • Materials
  • Proteins
  • Rodents
  • Sediments
  • Serodiagnosis
  • Sodium Hydroxide
  • Sugars

Fields of Study

  • Biology

Readers

  • Immunology
  • Spectroscopy.