GENETIC ANALYSIS OF MICROORGANISM BY MIXED INFECTION OF ACTIVE PHAGE PARTICLES AND PHAGE OR BACTERIAL DNA.
Abstract
The phage mediated conversion (or lysogenic conversion) of salmonella 0(1) antigen by a sub 1 phage could be changed into the DNA mediated transformation when Sg cells are incubated with purified DNA extracted from a sub 1 phage and active particles of a sub 10s phage. As far as the resuspension of such mix infected Sg cell is directly examined before lysis for the agglutinability to the anti-0(1) factor serum, a small proportion of them is shown to be really converted into the one shared with 0(1) antigen. However the converted character is almost confined in the generation of such converted cells so that it is scarcely inherited into their progeny cells. On the other hand, such 0(1) antigen conversion could not be seen upon the mix infection with a sub 1 DNA together with a sub 2 phage particles which is incompatible with a sub 1 phage in the growth at the same host cells, or upon mix incubation with a sub 1 phage DNA alone. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 29, 1965
- Accession Number
- AD0624749
Entities
People
- Isamu Kondo