NITROGENOUS SUBSTANCE CHANGES IN THE NUTRITIVE BROTHS PREPARED FROM THE DRY KPD PREPARATION FOLLOWING CULTURING OF CL. BOTULINUM OF THE A, B, C AND E TYPES
Abstract
By using the standard medium prepared from the dry KPD preparation a study was made of the changes of chemical indices of nitrogenous substances in this medium resulting from the growth of and toxin formation by C1. botulinum of the A, B, C and E types. As demonstrated, the quantity of nitrogenous substances of the medium (amine nitrogen, peptone, tryptophane and of some other amino acids) changes characteristically for each of the Cl. botulinum types and in accordance with the extent of its proteolytic intensity. Amine nitrogen content increases in growing Cl. botulinum of the A and B types 3-fold, of the C types -- 2-fold, and of the E type - 1 1/2 times. The peptone level in the medium also goes down inversely to the amine nitrogen content. Trytophane completely disappears while culturing A and B types, is halved in the growing of the C type and remains almost unchanged with the type E.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1965
- Accession Number
- AD0625617
Entities
People
- L. G. Ivanova
- T. I. Sergeyeva