DEVELOPMENT OF THEORY TO UNIFY MECHANISMS OF ENZYME CATALYSIS AND DENATURATION.
Abstract
Study of the denaturation of prostatic acid phosphatase was continued. Denaturation of the enzyme with ultraviolet radiation over a range of carefully controlled temperatures from -30 to 50C. was carried out in specially designed apparatus. The denaturation rate was also measured using urea or n-propanol in combination with ultraviolet radiation. Comparison was made with rates obtained using urea with the addition of other chemical agents already investigated: guanidine-hydrochloride, sodium p-toluene sulfonate, and n-propanol. The effect on the denaturation rate of acid phosphatase was also measured using two series of chemical compounds: chlorides of the alkali metals and methylated urea compounds: symmetrical dimethyl urea and tetramethyl urea. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 30, 1965
- Accession Number
- AD0627062
Entities
People
- Morris London
Organizations
- North Shore University Hospital