INTERACTIONS BETWEEN CORTISOL OR CORTICOSTERONE AND FRACTIONS OF RAT THYMUS, BRAIN AND HEART CELL,
Abstract
Interactions between (4-14C) cortisol or (4-14C) corticosterone and fractions of thymus, brain and heart cells of normal rats have been studied by multiple equilibrium dialysis; animals of different age, ranging from weaned (20 days of age) to adult (45 days) to old rats (100 days and over) have been used. Binding between the steroid hormones and total homogenates of thymus, brain and heart of the rat has also been investigated. The combining affinity for the two steroids with cytoplasmic fractions is greater than with nuclear fractions, especially for thymus and heart cell. The binding of corticosterone with all the fractions and the total homogenates obtained from animals of different age is higher than that observed with cortisol. A characteristic pattern showing a low binding affinity for 45 days old rats is observed in the interaction between the steroid hormones and some fractions for animals of different age. No accumulation of radioactive material was found in any particular fraction of the tissues investigated. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 17, 1965
- Accession Number
- AD0627367
Entities
People
- Frankc DeVenuto
- Gerald Chader
Organizations
- United States Army Medical Research Laboratory