RADIATION DAMAGE IN MUSCLE MEMBRANES AND REGULATION OF CELL METABOLISM.
Abstract
The report deals with studies of K and Cs ion effects on Na ion efflux and 02 consumption in resting muscle cell; and the action of trypsin and phospholipase C in the presence of Cs or K Ringer's solution on resting membrane potential. Na efflux results are consistent with calculated differences in penetration rates of C and K ions. But 02 consumption results are reversed from anticipated if considered a reflection of energy to drive the Na pump mechanism. Work has been done on membrane depolarization by various agents and their interaction and reversibility characteristics. Acetylcholine (Ach), Ach-Cs depolarizes membrane approaching a value of 55 mv. or approximately the threshold action potential of membrane. This may be reversed by displacement by washing with K-Ringer. It is prevented by d-tubocurarine but not eserine. Trypsin has a stronger effect on Cs than K treated membrane. Tr does not alter capacity of Ach to evoke depolarization but alters its reversibility when Cs is substituted for K. A model receptor system and allosteric action mechanisms are discussed. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1965
- Accession Number
- AD0629215
Entities
People
- Juan Carlos Perez
- Peter Stewart
Organizations
- University of Buenos Aires