THE INCORPORATION OF TRITIUM FROM THYMIDINE INTO PROTEINS OF THE MOUSE,
Abstract
Tritium from methyl-3H-Thymidine was found to be incorporated into proteins in mice. This incorporation in the mouse as a whole represented between 1 and 10% of the injected tritium. Tritiated water was not responsible for the labeling. Transmethylation reactions were proposed as a means whereby certain amino acids might have acquired the tritium from thymidine at some stage of its catabolysis. The initial (2-hour) ratios of DNA to protein tritium activities per mg wet tissue ranged from 5 in two tissues of low DNA synthetic activity (pancreas, liver) to 35-40 in two tissues of high DNA synthetic activity (spleen, small intestine). Labeled nuclear protein was coincident with labeded DNA in nuclei, where it constituted less than 2.5% of the total tritium. The significance of the findings was discussed. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 21, 1966
- Accession Number
- AD0630331
Entities
People
- Bernard J. Bryant
Organizations
- Naval Radiological Defense Laboratory