THE DNA CONTENT OF MITOTIC NUCLEI OF TWO MOUSE TUMORS,
Abstract
A new high-precision microspectrophotometric method was used to test the validity of the 'DNA constancy hypothesis' in two mammalian tumor lines. The two are the mouse ascitic tumor BP-8 and the in vitro mouse leukemic cell line, L5178Y. It was shown that the mitotic nuclei of these cells have a DNA content with a coefficient of variation of 5-6%. The instrumental and measurement errors are 4-5%; thus the variability of DNA content as measured by absorption of Feulgen stain is 1% or less. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 03, 1966
- Accession Number
- AD0631145
Entities
People
- E. L. Alpen
- M. E. Johnston
Organizations
- Naval Radiological Defense Laboratory