THE EFFECT OF DIPYRIDAMOLE ON THE ADENOSINE TRIPHOSPHATE LEVEL OF STORED HUMAN BLOOD.
Abstract
The addition of dipyridamole, a synthetic deaminase inhibitor at a concentration of .0001 M to human Heparin-ACD blood fortified with adenosine, resulted in an increase in ATP above control levels during storage at 4 C, rising to a maximum of 130% at five days and falling to 20% at 30 days. Aliquots of the same blood fortified with adenine, adenosine or both incubated at 37 C for 30 hours showed levels at or slightly above the upper limit of +2 SD of normal. The addition of dipyridamole at a concentration of .0001M to similar aliquots showed significant increases in ATP above normal, rising to a maximum of 300, 350 and 400% in the aliquots fortified with adenine, adenosine and both respectively. Whereas the presence of adenine, adenosine or both in Heparin-ACD blood did not significantly alter the rise in whole blood inorganic phosphate during incubation at 37 C for 30 hours, the addition of dipyridamole to similar aliquots depressed the level by from 20 to 30% of control values. Protection of the amino group of the adenine constituent of adenosine and AMP is a means to conserve erythrocyte ATP. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 30, 1965
- Accession Number
- AD0634018
Entities
People
- Fabian Lionetti
- John G. Gibson
Organizations
- Boston University