RESEARCH ON THE BASIS OF COLD INJURY.
Abstract
The objective of the research study was to design, develop, and fabricate instruments for an to conduct studies on the effect of controlled deionization during slow freezing on viability of biological tissues. The studies included but were not limited to: (1) nature of freezing injury to living cells and, (2) employment of the instruments to study the effect of freezing on blood. Macro- and micro freeze-electrodialysis instruments were designed, fabricated, and placed into operating condition. With the macro unit, controlled deionization during slow freezing under isotonic conditions was achieved initially with a model saline system and subsequently, with blood. The micro unit was employed to observe directly under the microscope the morphology of the erythrocytes during deionization and freezing. Results obtained with the blood samples indicated that improved stability to freezing was not realized with the electrodialysis treatment. Apparently, the passage of electrical current through blood during freezing promotes hemolysis. What is believed to be a significant contribution to the area of blood preservation was achieved when it was found that definitely improved stability to freezing is obtained by separating plasma from whole blood, concentrating and partially deionizing it, and then remixing the concentrated plasma with the erythrocytes. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1965
- Accession Number
- AD0634045
Entities
People
- Basil J. Luyet
- George J. Malecki