AN IMPROVED METHOD FOR THE RADIOISOTOPIC LABELING OF POLIOVIRUS AND VACCINIA VIRUS.

Abstract

The nucleic acid inhibitors, actinomycin D and 5-fluorodeoxyuridine (FUDR), were used to direct more radioisotopic label (P32 and C14) into viral nucleic acid. Phosphorus-starved HeLa cells were treated for 1 hour with 5 micrograms/ml. actinomycin D, infected with poliovirus, and then labeled with P32-dihydrogen phosphate. L cells were infected with vaccinia virus and DNA synthesis was immediately stopped by the addition of Fudr. C14-thymidine was added 6 hours after infection. It was found that actinomycin D increased the labeling efficiency by a factor of 20 and that FUDR increased it by a factor of 100. (Author)

Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1966
Accession Number
AD0634057

Entities

People

  • David J. Giron

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

DTIC Thesaurus Topics

  • Acids
  • Bacterial Infections And Mycoses
  • Diseases And Disorders
  • Efficiency
  • Infection
  • Inhibitors
  • Microorganisms
  • Nucleic Acids
  • Phosphorus
  • Poxviridae Infections
  • Thymidines
  • Viruses
  • Wound Infections

Readers

  • Solar Photovoltaics and Thermoelectric Devices.
  • Toxicology/Environmental Toxicology
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology