ISOLATION AND IDENTIFICATION OF TOXIC FRACTIONS OF COBRA VENOM
Abstract
The results of this study indicated that six physiologically active fractions can be separated from crude cobra venom by fractionization procedures. The administration of the first fraction produced a marked depression of the cortical electrical activity. It has been shown that crude cobra venom contains large amounts of phospholipase A. Although it has not been established at the present time it is felt that the change in EEG may not be the result of phospholipase A activity. Fractions 5-8, which may produce respiratory paralysis by blocking neuromuscular transmission at the diaphragm, appeared to do so by interfering with some action of acetylcholine. There are, however, others who indicated that this phenomenon may be the result of increased nerve membrane permeability. This concept is consistent with a proposed general breakdown of membrane phospholipid by venom, producing an over all depolarization and block rather than any specific inhibition. The effect of fraction 12 on arterial blood pressure and heart rate indicated that cobra venom has a definite action on the cardiovascular system of the dog.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1966
- Accession Number
- AD0634646
Entities
People
- Byron T. Currie
- Clarence Broomfield
- Henry P. Ciuchta
- James A. Vick
- James H. Manthei