EVALUATION OF TECHNIQUES FOR BIOASSAY OF ERYTHROPOIETIN IN SERA OF HUMANS AND ANIMALS IN AMBIENT AND HYPEROXIC ENVIRONMENTS.
Abstract
The regulation of erythropoiesis by blood pO2 is of direct consequence to any evaluation of the suitability of higher than ambient O2 partial pressures in space cabin environments. Alterations in serum erythropoiesis stimulating factor (ESF) of individuals exposed to space cabin environments for long periods of time may have clinical consequences. Four methods for the assay of serum ESF were evaluated, and of these, only the hypoxia-induced polycythemic bioassay rat proved to be sensitive enough to detect ESF in 2 ml of serum. Serum ESF is related to that fraction of a dose of Fe59, injected into the bioassay rat, which appears in the red blood cells after twenty-four hours. Endogenous ESF causes Fe59 uptakes of 6.4 plus or minus 3.9%; 2 ml of human serum causes Fe59 uptakes of 15.4 plus or minus 6.8%, which are statistically significant from the controls. Variances in duplicates are such that to distinguish between two samples at the 95% confidence interval, the samples must differ from each other by about 50% in ESF content. Although statistically significant changes in blood ESF were found, both in animals and humans exposed to 100% O2, the physiological significance of these changes is unexplained due to the paucity of data. The unique discovery that neuramin lactose, neuraminic acid, and beta-lactose stimulate Fe59 uptake into red blood cells would indicate that the parameters which control incorporation of iron into red blood cells need further definition. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1965
- Accession Number
- AD0635599
Entities
People
- Bernard J. Katchman
- George Beemsterboer
Organizations
- Miami Valley Hospital