ELECTROPHORETIC SEPARATIONS ON ACRYLAMIDE GELS: DISC ELECTROPHORESIS

Abstract

Disc Electrophoresis, a new method for fractionating serum proteins and enzymes developed by Ornstein and Davis (Ann. N. Y. Acad. Sci. v121 p321 1964), possesses great sensitivity, speed and reproducibility while requiring a sample as little as 3 microliters in routine separations. The technique as adapted and standardized for use in the Toxic Hazards Branch, Aerospace Medical Research Laboratories, for serum protein and Lactic Dehydrogenase (LDH) isozyme separations is presented along with suggestions for making the apparatus needed to perform disc electrophoresis.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1965
Accession Number
AD0637228

Entities

People

  • Leandro Rendon

Organizations

  • Air Force Research Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetic Acid
  • Air Force
  • Biomedical Research
  • Blood
  • Blood Proteins
  • Bromphenol Blue
  • Buffers (Chemistry)
  • Chemistry
  • Classification
  • Electrophoresis
  • Gels
  • Materials
  • Polymers
  • Power Supplies
  • Proteins
  • Security
  • Toxic Hazards

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Molecular and Cellular Biochemistry

Technology Areas

  • Space