DEFINED MAINTENANCE MEDIUM FOR SUPPORTING CHICK FIBROBLAST MONOLAYERS AND FOR PLAQUE FORMATION BY VENEZUELAN AND EASTERN EQUINE ENCEPHALITIS VIRUSES

Abstract

A defined maintenance medium that supports chick fibroblast (CF) cell monolayers in a viable state for at least 10 days and permits the formation of plaques by Venezuelan (VEE) and Eastern (EEE) equine encephalitis viruses is described. The medium consists of a balanced salts solution, glucose, 2 amino acids (L-cystine and L-histidine HC1), sodium bicarbonate, and agar. Plaque counts by VEE and EEE viruses on CF monolayers overlaid with this medium compared favorably with those obtained on a complex overlay medium.

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Document Details

Document Type
Technical Report
Publication Date
Aug 23, 1964
Accession Number
AD0638563

Entities

People

  • Eugene Zebovitz

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Acids
  • Albumins
  • Amino Acids
  • Aspartic Acid
  • Biological Laboratories
  • Carbon Dioxide
  • Cells
  • Chlorides
  • Encephalitis
  • Equine Encephalitis
  • Fibroblasts
  • Histidine
  • Indicator Dyes
  • Infectious Diseases
  • Magnesium Compounds
  • Sodium Compounds
  • Viruses

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Virology (or Medical Virology).