INDUCIBLE LYTIC SYSTEMS IN THE GENUS BACILLUS

Abstract

Many different strains of Bacillus cereus and members of other species of the genus Bacillus were induced to lyse by exposure to the proper concentration of mitomycin C, a radiomimetic drug. For some bacilli, induction of lysis occurred only in Casamino acids containing medium but not in nutrient broth+yeast extract medium, whereas other bacilli showed the opposite behaviour. The efficiency of induction was a function of the growth phase of the culture and of the concentration of mitomycin C. Induction of lysis in B. thuringiensis resulted in the release of oedema factor and phospholipase into the medium. Mitomycin C-induced lysis of B. cereus was prevented by adding chloramphenicol, actinomycin D, or 5-fluorouracil deoxyriboside up to 40 min. later. There appeared to be no sequential loss of susceptibility to inhibition by these compounds as expected.

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Document Details

Document Type
Technical Report
Publication Date
Oct 12, 1964
Accession Number
AD0638575

Entities

People

  • H. B. Stull
  • R. A. Altenbern

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Animals
  • Anti-Bacterial Agents
  • Bacteriophages
  • Biological Laboratories
  • Biomedical Research
  • Electron Microscopy
  • Extinction
  • Incubation
  • Inhibition
  • Inhibitors
  • Insect Control
  • Laboratory Animals
  • Production
  • Prophages
  • Radiation
  • Ribonucleic Acids

Fields of Study

  • Biology

Readers

  • Microbial Pathology