MATERIALS ON THE STUDY OF THE DRY TULAREMIA VACCINE PREPARED UNDER INDUSTRIAL CONDITIONS.
Abstract
Conclusions: For obtaining tularemia vaccine under industrial conditions it is possible to use the method of aeration of cultures in a vibrator. Tularemia microbes, incubated in a vibrator, are no different in their biological properties than microbes cultivated under the conditions of aeration through a diffuser. For the preparation of tularemia vaccine under industrial conditions with the utilization of the method of deep cultivation of the microbes it is possible to use semiliquid nutrient media with hydrolysate of whale flour, casein hydrolysate and Hottinger broth. For the purpose of preparing inoculation material for aeration it is possible to use agar media containing whale flour hydrolysate, fish hydrolysate and Hottinger broth instead of vitelline media. The greatest accumulation of microbes with a high content of immunogenic colonies is obtained on agar medium with Hottinger broth. The dry tularemia vaccine, obtained by the method of culture vibration, in the proposed semiliquid nutrient media conforms to the requirements set forth. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1965
- Accession Number
- AD0638578
Entities
People
- L. G. Tatomir
Organizations
- United States Army Biological Warfare Laboratories