INACTIVATION OF BACTERIOPHAGE BY ACRIDINE ORANGE
Abstract
A case is described of the dark inactivation of lambda phage by acridine orange. Dark inactivation is possible in solutions with a low ionic strength at a weakly alkaline pH. Inactivation follows the kinetics of a multi- hit process. The spectrum of the dye in the presence of lambda phage is changed in the same manner as the spectrum of the dye is changed in the presence of DNA. The lambda phage inactivated by the dye preserves the adsorption capability. The formation of the phage--dye complex, which leads to dark inactivation, is an irreversible process. Such a complex does not dissociate upon removal of the free dye. Apparently, injury to the sensitive structure is also irreversible: Removal of the dye does not restore the biological activity of the phage. The data presented testifies that the sensitive structure is the phage DNA and not the protein membrane.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1965
- Accession Number
- AD0638597
Entities
People
- V. K. Ravin
Organizations
- United States Army Biological Warfare Laboratories