THE SIGNIFICANCE OF TYPE E BOTULINUM TOXIN ACTIVATION IN THE LABORATORY DIAGNOSIS OF BOTULISM

Abstract

Conclusions: On artificial nutrient media, Cl. botulinum produces a toxin and a nontoxic prototoxin. The latter may be transformed into a toxin due to the action of various enzymes (pancreatin, proteinase of Cl. sporogenes). when botulinum prototoxin type E reaches the digestive tract its activation by enzymes takes place. As a result of this the toxic effect on the organism is strengthened. During the investigation of various materials (food products, discharges from a patient, section material, original seedings) for the presence of botulinum toxins, it is necessary to subject the nontoxic filtrates to activation by pancreatin, and after this set up biological tests and the neutralization reaction with antibotulinum serum type E.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 14, 1963
Accession Number
AD0639403

Entities

People

  • T. I. Bulatova

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Botulism
  • Clinical Laboratory Techniques (Medicine)
  • Epidemiology
  • Food Poisoning
  • Incubation
  • Lethality
  • Materials
  • Microbiology
  • Microorganisms
  • Military Medicine
  • Poisoning
  • Rodents
  • Russian Language
  • Toxicity
  • Ussr

Fields of Study

  • Medicine

Readers

  • Microbial Pathology
  • Molecular and Cellular Biochemistry