THE RESPONSE OF ERYTHROPOIETIC STEM CELLS OF MICE TO IRRADIATION WITH FISSION NEUTRONS,
Abstract
The destruction of erythropoietic stem cells by irradiation with fission neutrons was studied in plethoric C57 leaden male mice by measuring the incorporation of Fe59 into erythrocytes 48 hours after a dose of erythropoietin. The survival of stem cells in mice exposed to single doses of neutrons at 37 rad/min. followed the single-hit multi-target model of radiation injury to cells. D is dose, D sub o is 37% dose, and E is target multiplicity. D sub o was a constant, equal to 27.8 rads for all animals, but E varied from 1 to 4 in different animals. The survival of stem cells in mice exposed to single doses of neutrons at 1.75 rad/min. followed the single-hit multi-target model, with D sub o = 26 rad, for those animals for which E = 1, but animals with E>1 did not fit the model. The survival of stem cells in mice exposed to 3 doses at 37 rad/min. at intervals of 10-12 hours also followed the single-hit multi-target model, with mean D sub o = 26 rad, and a distribution of values of E similar to that in the single dose. The data strongly imply that the loss of stem cells follows the multi-target model, but that E. instead of being a constant, is a basic biological variable of the response. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 19, 1966
- Accession Number
- AD0640316
Entities
People
- J. S. Krebs
Organizations
- Naval Radiological Defense Laboratory