CATHODE LUMINESCENCE OF NORMAL AND CANCEROUS CELLS
Abstract
A method was developed for determining the antioxidative effect in culture cells and cells prepared by means of the trypsinization of animal tissues, based on the dying out of luminescence which accompanies the free radical processes at the cathode during the passage of direct current with a density of 200 ma/sq cm through a suspension of cells. It was established that cancerous cells, prepared by means of trypsinization of cancer of the stomach and cancer of the mammary glands, and also culture cells of a cancerous origin: Hela, Hep-2, CaPa, CaMa -- lose luminescence more intensively than normal cells, prepared by trypsinization of monkey kidneys, lungs of a 3--5 month old human embryo, and 9-day chick embryos. Based on their antioxidative effect, the culture cells of non-cancerous origin (sots, strain 580, cells from an upper limb of a 3-month old human embryo) approximate those of cancerous cells (Hela, Hep-2, and others).
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 12, 1965
- Accession Number
- AD0641750
Entities
People
- B. N. Tarusov
- V. S. Pyatenko
Organizations
- United States Army Biological Warfare Laboratories