AN ABSORPTION SPECTROPHOTOMETER CELL FOR USE AT HIGH PRESSURES AND ITS APPLICATION TO AN ENZYMIC REACTION.

Abstract

A cell designed for absorbancy measurements at high pressures has been constructed and used for a study of the pressure dependence of a thrombincatalyzed hydrolysis. Thrombin is not inactivated at pressures up to 20,000 psi. The rate of hydrolysis of a synthetic substrate (toluene sulfonyl arginine methyl ester) increases appreciably with pressure. (Author)

Document Details

Document Type
Technical Report
Publication Date
Nov 07, 1966
Accession Number
AD0641910

Entities

People

  • Robert F. Steiner

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Absorption
  • Blood Coagulation Factors
  • High Pressure
  • Hydrolysis
  • Measurement
  • Measuring Instruments
  • Pressure Measurement
  • Spectrophotometers
  • Substrates

Readers

  • Fluid Dynamics.
  • Molecular and Cellular Biochemistry
  • Organic Chemistry