PECULIARITIES OF ABSORPTION SPECTROPHOTOMETRY OF BIOLOGICAL OBJECTS. 2
Abstract
Conclusions: In an optical respect biological objects are distinguished by their heterogeneity. An uneven distribution of absorbing substance in an object always leads to a decrease of the absorption coefficient in comparison with the coefficient of the corresponding hemogeneous object. The absorption coefficient decreases most noticeably in the area of strong absorption. As a result of 'leveling' of the absorption spectrum takes place. The influence of light scattering on the absorption spectrum depends on the scattering and absorbing capabilities of the substance of the object, on their distribution in the object, and on the direction of incident light. If the scattering and absorption are not very great (so that a significant part of the rays penetrates into the depth of the object), then scattering most rapidly of all will lead to an increase of the absorption coefficient. With a very strong scattering, when a noticeable part of the rays scatter reversely and therefore do not cover a significant path in the object, and also during a strong absorption when the lengthening of the optical path of the ray within the object hardly influences the absorption coefficient, scattering may lead to a certain lowering of the absorption coefficient. Thus, under the influence of scattering a leveling of the absorption spectrum may also take place.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1965
- Accession Number
- AD0642026
Entities
People
- L. N. Bell
Organizations
- United States Army Biological Warfare Laboratories