INFLUENCE OF THE IONS OF TRIVALENT IRON ON THE PHYSICAL-CHEMICAL PROPERTIES OF SOLUTIONS OF NATIVE DESOXYRIBONUCLEIC ACID

Abstract

An investigation was made on the influence of the addition of Fe(3+) on the viscosity, dispersion curves of optical rotation and the absorption spectra of solutions of native DNA. It was shown that with the formation of a complex of DNA with Fe(3+) a lessening takes place in the values of the quoted viscosity in a wide range of concentrations of DNA. In the presence of Fe(3+ ions an increase is observed in the value of the specific rotation and a decrease in the absorption coefficient of solutions of DNA, and also an increase of the melting temperature in comparison with the melting temperature of pure DNA. The experimental data obtained agree with the model of a macromolecule of DNA, having a certain number of points at which there is the possibility for the rotation of parts of the molecule which are stable rods. The addition of Fe(3+) ions leads to an increase in the rigidity of the DNA macromolecule, by reducing the number of places around which a bending of the macromolecule takes place.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1966
Accession Number
AD0642071

Entities

People

  • A. N. Ilina
  • S. Y. Mirlina
  • V. D. Zhdanova
  • Yu S. Moshkovskii

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Absorption
  • Absorption Coefficients
  • Absorption Spectra
  • Acids
  • Aqueous Solutions
  • Biopolymers
  • Chemical Properties
  • Coefficients
  • Dispersions
  • Experimental Data
  • Macromolecules
  • Materials
  • Molecules
  • Nucleic Acids
  • Rotation
  • Russian Language
  • Viscosity

Fields of Study

  • Chemistry

Readers

  • Materials Science and Engineering.
  • Molecular and Cellular Biochemistry