REACTIVATION BY 2-PAMCL OF INHIBITED CHE ACTIVITY IN DOGS POISONED WITH PINACOLYL METHYLPHOSPHONOFLUORIDATE (SOMAN, GD)
Abstract
The effect of varying concentrations and dosages of 2-pyridine aldoxime methochloride (2-PAMCl) on the aging of cholinesterase (ChE) phosphonylated by soman in vitro and in vivo in dogs was studied. The rate of aging of dog red-blood-cell (RBC) ChE inhibited by soman in vitro and in vivo can be markedly diminished by 2-PAMCl, which reactivates the unaged portion of the inhibited ChE enzyme. Measurements of brain- and diaphragm-ChE activity in dogs poisoned with soman and treated with 0.104 gm/kg of 2-PAMCl showed that reactivation occurred only in the latter tissue. The close correlation found between in vitro and in vivo rates of aging suggests that, in a given species, estimation of the rate of aging of RBC ChE in vitro following inhibition with an organophosphate could determine the time during which oxime therapy would be effective in vivo against intoxication by the same anti-ChE.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1967
- Accession Number
- AD0645825
Entities
People
- Edmund F. Murtha
- Joseph H. Fleisher
- Larrel W. Harris