EVALUATION OF IMMUNOASSAY METHODS FOR PROLACTIN IN BODY FLUIDS

Abstract

A practical immunologic assay to detect the hormone, prolactin, in body fluids was evaluated. Prolactin content was assayed using positive antiprolactin sera prepared in rabbits and test samples of serum, urine, and urinary extract from a borane exposed female monkey in micro-Ouchterlony gel diffusion and agglutination inhibition tests. The gel diffusion test gave qualitative results while the agglutination inhibition test allowed quantitation of prolactin content. Results of the later test indicated a possible difference in pre- and postborane exposure prolactin content of monkey urine. Further work in this area will need to be done to clarify this preliminary evidence and to expand on its applicability to human exposure to boranes.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1966
Accession Number
AD0646432

Entities

People

  • D. A. Dilley

Organizations

  • Air Force Research Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Agglutination
  • Air Force
  • Antibodies
  • Antigen Antibody Reactions
  • Bioassay
  • Biochemistry
  • Blood
  • Blood Cells
  • Body Fluids
  • Cells
  • Centrifuges
  • Chemical Synthesis
  • Chemistry
  • Rodents
  • Sodium Compounds
  • Standards
  • Tannic Acid

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Cellular and Molecular Pathways of Apoptosis.
  • Toxicology/Environmental Toxicology