DNA SYNTHETIC RATE AND DNA CONTENT OF NUCLEATED ERYTHROID CELLS,
Abstract
Measurements of relative DNA content and DNA synthetic activity have been made on individual cells by autoradiographic measurement of C14-thymidine incorporation and microphotometric estimation of Feulgen reactivity on the same cells. It has been shown that mean DNA sythetic rate, as measured by label incorporation, does not vary during the synthetic phase; however, at the beginning and end of the S period average synthetic rate is reduced. The variability in individual grain counts is not reduced by classification of the cells according to location in the S period. It has also been shown that G sub 1 does not appear to exist for dog bone marrow normoblasts and that some cells appear to synthesize DNA without incorporation of the labeled precursor. There is evidence provided that DNA synthesis may be to a certain extent an intermittent process depending upon the chromosome replication patterns in individual nuclei. A model of nuclear DNA replication is presented which is consistent with the observed facts. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 02, 1967
- Accession Number
- AD0647036
Entities
People
- Edward L. Alpen
- Muriel E. Johnston
Organizations
- Naval Radiological Defense Laboratory