EFFECT OF HYDRAZINES ON VITAMIN B6 LEVELS IN THE MOUSE BRAIN.
Abstract
The effects of administered 1,1-dimethylhydrazine (UDMH) and monomethylhydrazine (MMH) on vitamin B6 levels in mouse brain have been studied. Separation of the B6 group (pyridoxol, pyridoxal, pyridoxamine, and the respective 5-phosphates) by means of paper chromatography revealed that the R sub F values obtained are dependent upon the pH of the developing solvent. To obtain the time lag just prior to convulsions induced by UDMH and MMH, a dose-lag time study was conducted; included were pyridoxal and (its 5-phosphate) hydrazones of UDMH and MMH. Graphs of log dose vs lag time are given. The bioassay procedure, though not completed, permits detection of some of the B6 congeners to a limit of 0.5 nanograms. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1966
- Accession Number
- AD0647192
Entities
People
- Arthur Furst
- Waldemar R. Gustavson
Organizations
- University of San Francisco