ELECTRON MICROSCOPE STUDY OF THE INFECTIOUS AND SERUM HEPATITIS.
Abstract
An examination was made of several enzyme activities of the hepatocytes infected with infectious or serum hepatitis virus particles at the electron microscopic level. This was done for two reasons: (a) to ascertain whether certain enzyme activity is located in the inner nucleoid or the outer membrane of such particles; and (b) to examine whether the virus-like particles are found in association with cellular components showing certain enzyme activity. Prior to making an examination of enzyme reaction on the biopsy material, several enzyme reactions were tried on rat normal and regenerating liver cells, because it is important to consider fixation procedure to reserve enzyme activity without doing damage to the cytoplasmic structure. The aim of this investigation was to look into the localization of glucose-6-phosphatase (G-6-Pase) at a fine structural level in rat liver, which is a specific phosphatase hydrolyzing either glucose-6-phosphate or glucose-1-phosphate, as glycogen is converted to glucose. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1967
- Accession Number
- AD0647583
Entities
People
- Gonpachiro Yasuzumi