INFLUENCE OF THE RELATION BETWEEN THE PROTECTIVE MEDIUM AND THE MICROBIAL SUSPENSION ON THE VIABILITY OF THE EV VACCINE DURING DRYING AND STORAGE
Abstract
The work was conducted for the purpose of studying the influence of the relationship of the volumes of protective medium and native suspension on the survival rate of the bacteria during the process of drying and storage of the vaccine. In the test 12 series of vaccine were used. Each test series was tested in three variants: Variant I -- to three volumes of native suspension one volume of protective medium was added; Variant II -- to three volumes of native suspension two volumes of protective medium were added; Variant III -- to three volumes of native suspension three volumes of protective medium were added. All the variants were checked for survival before drying, immediately after drying, and after seven months of storage at refrigerator temperature. The results showed that the most significant lowering in the indices of survival were noted for variant III of the tests (by 23% following drying and 32% following prolonged storage, as opposed to the corresponding indices of 15 and 32% for variant II and 16 and 27% for variant I). This investigation makes it possible to conclude that the dilution of a native suspension of EV antiplague vaccine with large quantities of protective medium lowers the survival rate of the bacteria during the process of drying and the subsequent storage of the vaccine.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1966
- Accession Number
- AD0648107
Entities
People
- L. S. Sviridova
Organizations
- United States Army Biological Warfare Laboratories