COMPARATIVE CYTOFLUOROMETRIC STUDY OF THE DNA CONTENT IN HIGH POLYPLOIDY CELL NUCLEI STAINED WITH ACRIDINE ORANGE AND FLUORESCENT SCHIFF-TYPE REAGENT

Abstract

An investigation of the macronucleus of Tetrahymena pyriformis, stained with fluorescent Schiff-type reagent (auramine 0-SO2) in the Feulgen reaction, showed that the cytofluorometric method may be used to determine the relative amounts of DNA even in high polyploidy cellular nuclei. When RNA has been removed from the cells it is possible to judge the condition of the DNA-protein based on measurements of the intensity of fluorescence of the cell nuclei, stained with acridine orange. The two methods are complementary in the investigation of cell nuclei by the cytofluorometric method.

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Document Details

Document Type
Technical Report
Publication Date
Nov 24, 1964
Accession Number
AD0651175

Entities

People

  • A. A. Zotikov

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Acids
  • Acridines
  • Biology
  • Cell Nucleus
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Deoxyribonucleic Acids
  • Fluorescence
  • Health Services
  • Life Cycles
  • Microscopy
  • Molecular Biology
  • Nucleic Acids
  • Proteins
  • Ribonucleic Acids
  • Trna

Fields of Study

  • Biology

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Molecular Genetics