METHOD OF FLUORESCENT ANTIBODIES IN THE STUDY OF THE ANTIGENIC STRUCTURE OF BACTERIA
Abstract
The use of the fluorescent antibody method made it possible to expose the individual antigens of the microbial cell and to show their localization in specific organelles -- the capsule, membrane, and apparently the cytoplasm. The combined application of immunological methods and methods of cytochemistry made it possible to establish that the antigen complexes of various organelles are characterized by a specificity of antigen composition connected with their chemical differences. It was shown that microorganisms, possessing a capability to exist in various forms - capsular, capsule-less and spore, are characterized by an antigenic specificity, distinct for each of these forms. This example of the use of various complex methods for studying the microbial cell(fluorescent antibody method, methods of cytochemistry, luminescent and light microscopy, methods of disintegration of the microbial cell) is proof of the expediency and future possibilities of using these methods in studying the antigenic structure of microorganisms.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1966
- Accession Number
- AD0651236
Entities
People
- Ye N. Levina
Organizations
- United States Army Biological Warfare Laboratories