A STUDY OF BOTULIN TOXINS AND TOXOIDS BY FILTRATION THROUGH GEL. REPORT 1. EXAMINATION OF BOTULIN TOXINS AND TOXOIDS OF THE A AND C TYPES BY FILTRATION THROUGH SEPHADEX G-100

Abstract

By filtration through Sephadex G-100 dextran gel it is possible to separate type A and C botulin toxins and toxoids into fractions. The first fraction isolated by filtration through gel contained proteins with a molecular weight greater than 100,000; it corresponded to the antitoxin-binding activity of the toxoids and the partial lethal activity of toxins. Filtration through gel makes it possible to separate botulin toxins and toxoids into fractions which are different in their antigenic spectrum. By filtration of botulin toxoids through Sephadex gel it is possible to purify the active fractions from ballast low molecular compounds.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1966
Accession Number
AD0651379

Entities

People

  • V. B. Freiman
  • V. K. Golshmid

Tags

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Antitoxins
  • Aromatic Amino Acids
  • Blood Proteins
  • Buffers (Chemistry)
  • Chemistry
  • Clinical Laboratories
  • Complex Mixtures
  • Extinction
  • Filtration
  • Molecular Weight
  • Nitrogen
  • Nitrogen Compounds
  • Precipitation
  • Proteins
  • Spectra

Fields of Study

  • Computer science

Readers

  • Immunology
  • Microbial Pathology