EARLY DETECTION OF ARBOVIRUSES IN TISSUE CULTURES BY HEMAGGLUTINATION

Abstract

The hemagglutination reaction with the virus of Venezuelan encephalomyelitis and the Western variant of American equine encephalomyelitis, incubated in tissue cultures of chick fibroblasts, takes place at 4C in a pH zone of 5.4--6.6; optimum pH 5.8--6.0. During infection of the cultures with large doses of virus within the limits of 10 to the 7th power to 10 to the 9th power CPD50 the hemagglutinins appear in the cultural fluid in 5--6 hours following infection, and the cytopathic effect is detected after 24 hours. The hemagglutination phenomenon may be used for the early detection of a virus in cultures.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1966
Accession Number
AD0652673

Entities

People

  • S. Y. Gaidamovich
  • V. A. Vagzhanova

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Arbovirus Infections
  • Arboviruses
  • Biological Laboratories
  • Detection
  • Erythrocytes
  • Fibroblasts
  • Hemagglutination Tests
  • Infection
  • Materials
  • Russian Language
  • Tissue Culture
  • Translations
  • Virology
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Mathematics or Statistics
  • Virology (or Medical Virology).