FATIGUE AND STRESS STUDIES: AN IMPROVED SEMI-AUTOMATED PROCEDURE FOR FLUOROMETRIC DETERMINATION OF PLASMA CATECHOLAMINES,
Abstract
A semiautomated technique is described for the estimation of total catecholamines in plasma by the trihydroxyindole procedure. The method utilizes conventional alumina-column chromatography for isolation of the amines. Catecholamine oxidation, tautomerization, and fluorescence detection are accomplished by an automated chemical system. Recoveries from plasma of 0.01 and 0.02 micrograms total catecholamines/10-ml sample were 86.4% plus or minus 8.9% (SD) and 85.6% plus or minus 5.8% (SD), respectively. Total catecholamine level in resting human plasma, determined by the present technique, was 0.89 plus or minus 0.15 (SD) micrograms/l. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1966
- Accession Number
- AD0653748
Entities
People
- Vincent Florica
Organizations
- Civil Aeromedical Institute