THE EFFECT OF SOME PROTECTIVE SUBSTANCES ON THE PROCESS OF FREEZING AND THAWING OF CELLULAR ELEMENTS IN THE BLOOD.
Abstract
Damage to blood cells in blood taken with the usual glucose-citrate solution with out the addition of protective substances, occurs during the process of freezing and thawing, apparently, occurs essentially during the stage of thawing. Ethyl alcohol, used as a protective substance, in a complex glucose-citrate solution, protects to a certain degree, the blood cells during the process of freezing and thawing in spite of intensive re-crystallization, even at relatively low temperature. In the concentrated solution, containing two protective substances (ethyl alcohol and glycerine), the process of recrystallization is weak. At temperatures to -50C a considerable portion of the solution remains non-crystallized and fills up the canals between the crystals in which there are present some mobile cells. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 11, 1967
- Accession Number
- AD0658850
Entities
People
- G. A. Kozlov
- V. I. Teodorovich