BIOLOGIC REACTIONS OF CELLULAR ANTIBODIES WITH SPECIAL REFERENCE TO THEIR IMMUNO-PATHOLOGICAL AND IMMUNO-CHEMICAL PROPERTIES
Abstract
As previously described, the cells, cultivated from the omentum of BSA-sensitized rabbits and grown in a special culture medium, were shown to contain antibody to the antigen, as detected by the fluorescent antibody technique. The cells were possibly originated in the reticulum cells and termed 'omentum cells'; and they seemed useful for the study of cell-bound antibody. The cell-bound antibody was separated from the cell extracts or culture fluid medium and partially purified by fractionation with ammonium sulfate followed by gel filtration with Sephadex G-200 or by sucrose density gradient centrifugation; and the presence of antibodies of 7S and 19S classes was confirmed. The cell-bound 19S antibody was found to have a characteristic ability to sensitize the homologous skin (rabbit skin) and to cause intensively the passive cutaneous Arthus reaction and reversed passive Arthus reaction in rabbit skin. Such biologic activity was found apparently less marked or negligible when the cell-bound 7S antibody or serum 7S and 19S antibody were assayed similarly in the rabbit skin. Further purification of cell-bound 19S antibody is being advanced.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1967
- Accession Number
- AD0660325
Entities
People
- Hideo Hayashi
Organizations
- Kumamoto University