STUDY OF THE EFFECTS OF OXYGEN TENSION ON OSTEOGENESIS.
Abstract
The purpose of this research project is to investigate the role of oxygen in osteogenesis. It was necessary to develop a method which would allow for the direct quantitative measurement of oxygen in the extracellular fluid of living bone cells. The system developed for this purpose is the oxygen microelectrode ear chamber. The results indicate that there is an overall reduction of oxygen tension in the part of the chamber occupied by the living bone culture. There is also a reduction in oxygen tension of the chamber as a whole compared with that prior to the introduction of the graft. It is concluded from the results that the ear chamber is a reliable, reproducible system with respect to quantitating directly gas tensions of in vivo cultures and their surrounding media. It is uniquely suitable for studying at the cellular level the diffusion of gases in viable calcified tissues. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1967
- Accession Number
- AD0660445
Entities
People
- H. C. Ezra
Organizations
- University of Oxford