EFFECT OF WHOLE-BODY IONIZING IRRADIATION ON ACTIVITY LEVELS OF BLOOD SERUM ENZYMES
Abstract
The purpose of this study was to review the technical literature to obtain information on whether whole-body exposure of animals to ionizing radiation in the approximate LD50 dose range for man (300 to 600 rads) induces changes in the activities of specific blood serum enzymes that are dose-related and suitable as a basis for the development of a biological radiation dosimeter. Of the enzyme systems reviewed, lactic dehydrogenase (LDH) isoenzymes and cholinesterase appear to have the most promise. Values obtained for the ratio of LDH isoenzyme bands 1:2 showed that various levels of ionizing radiation, including the LD50 range for man, can be distinguished. At 24 hr after sham irradiation the relative ratio of bands 1:2 of normal (control) male rhesus (Macaca mulatta) monkeys was in the range of 1.0 to 2.5, whereas at 24 hr after 200 to 500 rads of gamma irradiation of monkeys the ratios ranged from 0.88 to 0.36. At radiation levels above 500 rads, the LDH isoenzyme response was further magnified. The serum cholinesterase activities of various species of animals exposed to whole-body ionizing persisted over a 2- to 3-day period. Although this observation was based on a relatively limited number of evaluations, the response was consistent.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1967
- Accession Number
- AD0664176
Entities
People
- Albert Weinstock
- E. J. Hawrylewicz
Organizations
- IIT Research Institute