INHIBITION BY METHYLPHENIDATE OF TRANSPORT ACROSS THE YEAST CELL MEMBRANE.
Abstract
Methylphenidate at a concentration of 10 mM inhibited CO2 production and O2 uptake of yeast cells by 75%. This effect, with glucose as a substrate, occurred at pH 7.0, but not at pH 4.5. Kinetic data indicated the reaction was complex, and after binding, methylphenidate could not be readily removed. Glycolysis by cell free extracts was not inhibited at the 10 mM concentration, but was affected at 100 mM. O2 utilization with maltose and ethyl alcohol as substrates also was reduced. Entry into the cells of a number of different carbohydrates and glycine was inhibited to different degrees. The loss of 280 millimicron absorbing materials from suspended cells was reduced and the efflux of sorbose, arabinose and lactose was decreased. Thus, transport into and out of the cells was inhibited, and leakage, or permeability, was reduced. A general reaction was a cell membrane constituent or constituents is indicated. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 08, 1967
- Accession Number
- AD0664761
Entities
People
- Edward Spoerl
- R. J. Doyle
Organizations
- United States Army Medical Research Laboratory