THE APPLICATION OF SELECTIVE ULTRAFILTRATION TO PROTEIN CHEMISTRY

Abstract

Preformed ultrafiltration membranes, with molecular weight exclusion limits varying from 1,000-100,000, are uniquely suited for preparative methods in protein chemistry. The applications include non-denaturing protein concentration, rapid dialysis, micro solute binding, limited purification, and fractionation of complex mixtures. In the latter procedures, selective ultrafiltration obviates the use of cumbersome electrophoretic methods, or initial column chromatography. Increases in the separation rate, as well as the total quantity of material fractionated, can be obtained by increasing the exchangeable surface of the filter.

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Document Details

Document Type
Technical Report
Publication Date
Jan 15, 1968
Accession Number
AD0666254

Entities

People

  • William F. Blatt

Organizations

  • United States Army Research Institute of Environmental Medicine

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Biochemistry
  • Biomedical And Dental Materials
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Column Chromatography
  • Complex Mixtures
  • Filtration
  • Materials
  • Molecular Weight
  • Polymer Chemistry
  • Polymeric Films
  • Proteins
  • Proteomics
  • Ultrafiltration
  • United States

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Systems Analysis and Design